primary antibodies against ccnd1  (Boster Bio)

Journal: Journal of Orthopaedic Surgery and Research

Article Title: B-doped nano-hydroxyapatite facilitates proliferation and differentiation of osteoblasts

doi: 10.1186/s13018-024-05414-3

Figure Lengend Snippet: Sequences of primers

Article Snippet: Then, primary antibodies against CCND1 , MYC , PCNA , ALPL , and RUNX2 (1: 1000, Wuhan Boster Biological Technology Co., Ltd., Cat. No. AF1011, AF0511, AF0921, AF0531, AF0591, respectively) and secondary antibodies (goat anti-rabbit IgG conjugated with HRP, 1:5000, Wuhan Boster Biological Technology Co., Ltd., Cat. No. BA1054) were added for membrane incubation, followed by color development with ECL reagent (TransGen Biotech Co., Ltd., Beijing, Cat. No. SQ201).

Techniques:

Journal: Journal of Orthopaedic Surgery and Research

Article Title: B-doped nano-hydroxyapatite facilitates proliferation and differentiation of osteoblasts

doi: 10.1186/s13018-024-05414-3

Figure Lengend Snippet: Effects of B-nHAp on genes related to cell proliferation and differentiation. A MRNA expressions of CCND1 , MYC , and PCNA, marker molecules of cell proliferation determined through RT-qPCR, B MRNA expressions of ALPL and RUNX2, marker molecules of cell differentiation, measured via RT-qPCR, and C marker molecules of cell proliferation and differentiation at the protein expression level determined by Western blotting. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Then, primary antibodies against CCND1 , MYC , PCNA , ALPL , and RUNX2 (1: 1000, Wuhan Boster Biological Technology Co., Ltd., Cat. No. AF1011, AF0511, AF0921, AF0531, AF0591, respectively) and secondary antibodies (goat anti-rabbit IgG conjugated with HRP, 1:5000, Wuhan Boster Biological Technology Co., Ltd., Cat. No. BA1054) were added for membrane incubation, followed by color development with ECL reagent (TransGen Biotech Co., Ltd., Beijing, Cat. No. SQ201).

Techniques: Marker, Quantitative RT-PCR, Cell Differentiation, Expressing, Western Blot

Journal: Molecular Therapy. Nucleic Acids

Article Title: Shrimp miR-34 from Shrimp Stress Response to Virus Infection Suppresses Tumorigenesis of Breast Cancer

doi: 10.1016/j.omtn.2017.10.016

Figure Lengend Snippet: The Mechanism of Shrimp miR-34 in Inhibiting Breast Cancer Progression (A) The prediction of genes targeted by shrimp miR-34. The seed sequence of miR-34 is underlined. (B) The direct interaction between shrimp miR-34 and its target genes (i.e., CCND1 , CDK6 , CCNE2 , E2F3 , MET , or FOSL1 ) in breast cancer cells (MDA-MB-231). Luciferase activity was normalized to the ratio of firefly and Renilla luciferase activities. (C) The effects of shrimp miR-34 expression on the expression of miR-34 target genes in breast cancer cells (MDA-MB-231 and MDA-MB-435). (D) The influence of shrimp miR-34 expression on the expression of miR-34 target gene-encoding proteins in breast cancer cells. Protein levels were detected using western blot analysis. As a control, miR-34-scrambled was included in the transfection. Tubulin was used as a loading control. (E) The loading of shrimp miR-34 onto the human Ago2 complex. Shrimp miR-34 was incubated with the human Ago2 complex, followed by EMSA. Shrimp miR-34 was visualized by ethidium bromide staining (top). Human Ago2 protein was detected with western blot analysis (bottom). The wedge indicates the concentration gradient of human Ago2 complex. (F) A model for the role of miR-34-mediated cancer cell growth and metastasis. In all panels, statistically significant differences between treatments are represented with asterisks (error bar, SD; *p < 0.05 and **p < 0.01).

Article Snippet: The paraffin sections were then incubated with primary antibodies against human CCND1, CDK6, CCNE2, E2F3, or MET (Proteintech Group, USA) at 4°C overnight, followed by incubation with HRP-conjugated goat anti-Rabbit IgG (Sigma-Aldrich, USA) for 2 hr at room temperature.

Techniques: Sequencing, Luciferase, Activity Assay, Expressing, Western Blot, Control, Transfection, Incubation, Staining, Concentration Assay